Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.

《熒光雜志》是一個國際論壇，用于出版同行評審的原創文章，推進這一既定光譜技術的實踐。所涉專題包括理論/和/或數據分析方面的進展、對芳香分子的光物理、溶劑和環境影響的研究、研制固定或時間分辨的測量方法、熒光顯微鏡、成像、光波/恢復測量和/或細胞生物學研究的磷光方面的進展，化學生物學和熒光技術在流式細胞學/分析、免疫學、高通量篩選/藥物發現、dna序列/陣列、基因組學和蛋白質組學中的先進應用。典型的應用可能包括研究大分子動力學和構象、細胞內化學和基因表達。該期刊還發表了一些論文，描述了新型氟醚的合成和特性，特別是那些顯示出獨特的靈敏度和/或光學特性的化合物。除原創文章外，該期刊還出版評論、快速通訊、短訊、給編輯的信件、專題新聞文章、技術和設計筆記等。