Each topical issue, organized by a guest editor who is an expert in the area covered, consists solely of invited quality articles by specialist authors, many of them reviews. Issues are devoted to specific technical approaches with emphasis on clear detailed descriptions of protocols that allow them to be reproduced easily. The background information provided enables researchers to understand the principles underlying the methods; other helpful sections include comparisons of alternative methods giving the advantages and disadvantages of particular methods, guidance on avoiding potential pitfalls, and suggestions for troubleshooting.Methods focuses on rapidly developing techniques in the experimental biological and medical sciences.Some recent thematic issues covered these hot topics:? Chromatin Structure? Editing and investigating genomes with TALE and CRISPR? Tools and Methods for Cellular Localization and Measurements? Recent development in bioinformatics for utilizing omics data? Nucleic Acids Nanotechnology? Systems Biology with Omics Data? Autophagy? Spatial mapping of multi-modal data in neuroscience? Advancing the boundaries of molecular cellular pathology? NGS applications to translational research? Detection and Quantification of Proteins in Clinical Samples Using Mass Spectrometry

每一期專題由一位在該領域具有專家資格的客座編輯組織，僅由專業作者邀請的高質量文章組成，其中許多是評論文章。這些問題專門討論具體的技術方法，并強調對議定書的明確詳細說明，以便容易地加以復制。所提供的背景資料使研究人員能夠了解這些方法的基本原理;其他有用的部分包括提供特定方法的優點和缺點的替代方法的比較、避免潛在陷阱的指導和故障排除建議。方法著重于快速發展的實驗生物學和醫學技術。最近的一些專題討論涉及這些熱點問題:?染色質結構?使用TALE和CRISPR編輯和研究基因組?細胞定位和測量的工具和方法?利用組學數據的生物信息學的最新發展?核酸納米技術?帶有組學數據的系統生物學?自噬?神經科學中多模態數據的空間映射?推進分子細胞病理學的邊界?NGS在翻譯研究中的應用?使用質譜法檢測和定量臨床樣本中的蛋白質